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Proteomics International is
ISO 17025 ACCREDITED in proteomics services.
 
 

  Mass Spectrometry, Mass Spec, MALDI-TOF/TOF, Protein Identification by Mass Spectrometry, Tandem mass spectrometry - Proteomics International  

Mass spectrometry (MS/MS) sequencing MALDI or Electrospray
Mass spectrometry (mass spec) is concerned with the separation of matter according to atomic and molecular mass. 

High throughput mass spectrometry, using the most sophisticated instrumentation (TOF-TOF and LC/MS/MS) is exclusively used for precise analyses:
  • To identify any or all molecules
  • Differential expression
  • Full protein characterization
For protein sequencing by tandem MS (matrix-assisted laser desorption ionisation time-of-flight - MALDI-TOF/TOF or electrospray ionisation MS/MS), protein samples are enzymatically digested to produce fragmented peptides. Spectra are obtained for the major peptide ions in MS mode and sequence data obtained when the spectrometers automatically revert to MS/MS mode. These spectra are then compared to databases to provide hits that identify matching or similar sequences. The end result is positive verification of protein identity.
 
Analysis by MALDI-TOF/TOF (PMF+MS/MS) is optimal for Coomassie stained gels and 2D gel spots.
Analysis by electrospray (LC/MS/MS) mass spectrometry is optimal for gel bands containing two or more proteins or low abundant samples.
 
Fast, accurate results from services that are highly price competitive.
 
 
 
 
De novo peptide sequencing
Tandem mass spectrometry (MALDI TOF-TOF, or electrospray MS/MS) is used to determine the primary sequence structure of proteins and peptides that are not present in currently available databases. Crude extracts or pure samples can be analysed and our expert operators use the latest software to interpret the MS/MS spectra and derive your sequence.
 
For more information please refer to PI publication:
Bringans S.D., Kendrick T., Lui J., and Lipscombe R.J. (2008) A  comparative study of the accuracy of several de novo sequencing software packages for datasets derived by matrix-assisted laser desorption/ionisation and electrospray. Rapid Commun. Mass Spectrom. 22(21): 3450-4.

Request form De novo Peptide Sequencing Order Form & Pricing 
 
Peptide mass fingerprinting
Peptide mass fingerprinting for protein identification by mass spectrometry is now superseded by mass spectrometry  sequencing (PMF+MS/MS).