Circular Dichroism

Circular Dichroism (CD) spectrometry is used for secondary structure analysis of proteins (and other molecules which are chiral in structure).

For example the content of helix, b-sheet, random structure etc. can be determined within a protein. This technique is useful for measuring systems which undergo structural change such as the unfolding of proteins or helix formation in the presence of lipid etc. It is also a useful measure of determining if your protein is properly folded before entering other experiments such as NMR.

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